The NanoString nCounter digital molecular barcoding technology enables highly multiplexed, quantitative measurement of analytes of interest (DNA, RNA, miRNA, or protein) from a wide variety of sample formats with easy to use experimental protocols. The Applications team is adapting the cutting-edge technologies to be compatible with challenging biological sample types, including small amounts of FFPE or fresh frozen tissues and bodily fluids.
Your job as a summer intern is to help develop the protocols needed to make the new DNV single nucleotide variant (SNV) detection panel compatible with low-input samples such as whole blood and core needle biopsies. You will work with the team scientists to design mutant DNA-specific PCR primers to amplify rare mutant alleles and run experiments to fine tune and optimize the conditions. Toward the end of the project, you will have opportunities to test this new technology on circulating cell-free DNA extracted from whole blood from healthy individuals to prove the clinical utility of the new DNA SNV assay.
In parallel, you will also lead a project to test and optimize FFPE extract buffer for use with the nCounter Gene expression assay. In this project, you will perform FFPE extraction/purification and learn the nCounter Gene Expression assay workflow and data analysis.
What you will learn:
Our ideal candidate will be:
Our interns benefit from working in a collaborative team setting with talented coworkers who care deeply about the nanoString vision. We believe you will be able to quickly adapt to the fast-paced and creative environment of the biotechnology industry after finishing this summer project.
We are looking for an intern who can commit at least 25 hours a week for the duration of the Summer break. Submit your resume and cover letter, and we will be in touch if we think you are a good fit for us!